COVID-19 Diagnosed by Next Generation Sequencing (Explify)
Explify® Respiratory pathogen detection identified SARS-CoV-2 in an elderly patient presenting with fall-related trauma, lung opacities, and hypoxia.
An elderly man presented at a California emergency room with trauma-related injuries, including subdural hematoma and a radial fracture, suffered from a fall at his home. Pulse-oximetry indicated hypoxia. A chest CT scan revealed lung opacities with a ground-glass appearance. He was intubated for hypoxic respiratory failure and admitted to the ICU. A tracheal aspirate was sent for directed RT-PCR and to IDbyDNA for Explify Respiratory pathogen detection, which returned a strong positive result for SARS-CoV-2. 100% genome coverage was obtained with shotgun sequencing. Comparison with other sequences in public databases revealed the virus was most closely related to Clade G viruses with close similarities to other strains circulating in the United States. Unfortunately, the patient did not survive.